EN
3T3-L1 Mouse Embryonic Fibroblast (Pre-Adipocyte) Cell Line with LUC
Introduction
OriCell™ 3T3-L1 Mouse Embryonic Fibroblast (Pre-Adipocyte) Cell Line is a continuous subclone derived from the 3T3 cell line (Swiss albino mouse) through clonal isolation. Upon reaching confluence and undergoing contact inhibition after rapid proliferation, 3T3-L1 cells differentiate from preadipocytes into adipocyte-like cells. Under high-serum culture conditions, these cells accumulate intracellular lipids. As a well-established model for adipose metabolism research, 3T3-L1 preadipocytes are widely used to study adipocyte proliferation and differentiation.
OriCell™ 3T3-L1 Mouse Embryonic Fibroblast (Pre-Adipocyte) Cell Line with LUC is generated by transduction of the parental cells with a recombinant lentiviral vector encoding the luciferase gene. This modification results in stable luciferase expression, enabling efficient cell tracking and visualization both in vitro and in vivo.
When citing our products in academic publications, please use the following format: "OriCell™ [Product Name] + [Catalog Number], from Cyagen Biosciences."
Product Information
| Product Name | OriCell™ 3T3-L1 Mouse Embryonic Fibroblast (Pre-Adipocyte) Cell Line with LUC |
| Alternative Name | 3T3 L1 with Luciferase |
| Catalog Number | M5-0102 |
| Amount of Cells | 1×106 cells/vial |
| Tissue Origin | Mouse Embryo |
| Cell Characteristics | Adherent Growth; Fibroblast-like Morphology |
| Culture Conditions | 95% air; 5% CO2; 37 ℃ |
| Culture Medium | DMEM + 10% FBS |
| Doubling Time | 24 ~ 48 h |
| Passage Number | 1 |
| Storage at | Liquid Nitrogen (-196 ℃) |
| Precautions | If Luciferase expression is weak, add puromycin(0.5–2 μg/mL) for 2–4 days every 3–5 passages. Stop selection once the wild-type control cells died. |
QC
- Pass the detection of bacteria, fungi, mycoplasma, and endotoxins.
- Verified by cell recovery viability testing.
- Verified by STR analysis.
Please refer to "COA" for details.
General Handling Principles
- Maintain strict aseptic technique. Ensure complete sterility throughout all procedures, particularly within the laminar flow hood and incubator.
- Follow standardized protocols. Adhere strictly to the product manual. Implement rigorous control over experimental variables and include appropriate parallel controls.
- Use high-quality consumables and reagents. This product requires culture vessels suitable for adherent growth, and the reuse of these vessels is not recommended. The reagents used must be validated for reliability, cell compatibility, and batch-to-batch consistency.
Warm Notice: The cryopreservation medium of this product contains DMSO, which may pose potential risks. Please handle it with care.
