3D4/21 Porcine Alveolar Macrophage Cell Line

Introduction

The 3D4/21 Porcine Alveolar Macrophage Cell Line is one of the cell lines in the 3D4 series. It was derived from the alveolar macrophages of a 12-week-old Landrace pig and was spontaneously immortalized following transfection with the SV40 large T antigen. This cell line was obtained from the parental 3D4 cell line through single-cell cloning selection in the presence of G-418.

OriCell™ 3D4/21 Porcine Alveolar Macrophage Cell Line is mainly used to study the effects of puerarin on gene expression in the liver and gastrocnemius muscle of PEDV-infected piglets, as well as in 3D4/21 macrophages, and for gene expression profiling analysis of 3D4/21 cells infected with Mycoplasma hyopneumoniae.

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Product Information

QC

• Pass the detection of bacteria, fungi, mycoplasma, and endotoxins.
• Verified by cell recovery viability testing.
• Verified by STR analysis.

Please refer to "COA" for details.

General Handling Principles

• Maintain strict aseptic technique. Ensure complete sterility throughout all procedures, particularly within the laminar flow hood and incubator.
• Follow standardized protocols. Adhere strictly to the product manual. Implement rigorous control over experimental variables and include appropriate parallel controls.
• Use high-quality consumables and reagents. This product requires culture vessels suitable for adherent growth, and the reuse of these vessels is not recommended. The reagents used must be validated for reliability, cell compatibility, and batch-to-batch consistency.

Warm Notice: The cryopreservation medium of this product contains DMSO, which may pose potential risks. Please handle it with care.