Complete Medium For Monkey Bone Marrow Mesenchymal Stem Cells (Without EXO)

Introduction

Exosomes are a kind of vesicles secreted by cells to the outside of the cell, which contain rich contents, including lipids, nucleic acids, proteins, etc. Exosomes are widely found in various body fluids of mammals, including blood, lymph, milk, etc. FBS products for cell culture do not remove exosomes during the production process, so they cannot be used for exosome research in cells.

The serum component we used from the product (OriCell™ Complete Medium For Monkey Bone Marrow Mesenchymal Stem Cells, Cat. No.: MKXMA-90011) has undergone ultracentrifugation and multiple filtration steps to remove the exosomes. This process effectively retains the nutrients in the serum, making it suitable for cell culture. By culturing cells with this product, we can obtain abundant and high-purity exosomes.

When citing our products in academic publications, please use the following format: "OriCell™ [Product Name] + [Catalog Number], from Cyagen Biosciences."

Product Information

QC

• Pass the detection of bacteria, fungi, mycoplasma and endotoxins.
• Pass the detection of osmotic pressure and pH.
• Pass the detection of product quality.

Please refer to "COA" for details.

General Handing Principles

• Maintain strict aseptic technique. Ensure complete sterility throughout all procedures, particularly within the laminar flow hood and incubator.
• Follow standardized protocols. Adhere strictly to the product manual instructions. Implement rigorous control over experimental variables and include appropriate parallel controls.
• Ensure proper storage and use. Store all components according to specified conditions and use them promptly to ensure optimal performance.
• Aliquot for long-term storage. If the entire volume will not be used up immediately, prepare the medium in batches according to the specified component volume ratios and store in aliquots.

Product Stability and Storage Conditions

• All components must be stored away from light.
• The basal medium has a shelf life of 1 year and should be stored at 4 °C. Other components have a shelf life of 2 years and should be stored at -20 °C. 
• Once prepared, the medium has a shelf life of 1 month when stored at 4 °C. The shelf life may be extended up to a maximum of 45 days, provided that culture conditions remain stable, the container is properly sealed, and repeated temperature fluctuations are avoided.
• Use all components before the expiration dates. Expired components may severely compromise culture performance.