Osteogenic Differentiation Medium For Human Bone Marrow Mesenchymal Stem Cells

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Introduction

OriCell™ Osteogenic Differentiation Medium For Human Bone Marrow Mesenchymal Stem Cells, developed by the R&D team, contains a basic medium suitable for the growth of human bone marrow mesenchymal stem cells, OriCell™ Premium Fetal Bovine Serum, and a supplement for osteogenic differentiation of bone marrow mesenchymal stem cells.

This product is suitable for osteogenic induction and differentiation of human bone marrow mesenchymal stem cells from adults and fetuses. Extensive cell culture data have demonstrated that this product can induce these cells to differentiate efficiently and stably into osteoblasts.

When citing our products in academic journals, please indicate "OriCell™ + Catalog Number, from Cyagen Biosciences (Guangzhou) Inc."

Product Information

Components	Catalog Number	Volume
OriCell™ Basal Medium For Cell Culture	BLDM-03011	177 mL
OriCell™ Fetal Bovine Serum (Superior-Quality)	FBSSR-01021	20 mL
OriCell™ Supplements For Human Bone Marrow Mesenchymal Stem Cells Osteogenic Differentiation	HUXMX-04021	3 mL
Alizarin Red S Solution（pH=5.1~5.3）	ALIR-10001	10 mL
Gelatin	GLT-11301	10 mL

QC

Pass the detection of bacteria, fungi, mycoplasma and endotoxins.
Pass the detection of osmotic pressure and pH.
Pass the detection of product quality.

Please refer to "COA" for details.

General Handing Principles

Ensure that all equipment is kept clean and tidy.
Standard operation method. Please operate according to the method described in the product manual.
The ingredients should be properly stored in accordance with the storage conditions and used as soon as possible.
If complete medium cannot be used in a short period of time, it should be prepared in batches according to the volume ratio of each component in the kit and stored in aliquots.

Product Stability and Storage Conditions

All ingredients must be kept in dark place.
The basal medium should be stored in a refrigerator at 4°C for a period of 1 year. Other components should be stored at -20°C for a period of 2 years.
The prepared complete medium can be stored at 4°C for a period of 1 month. If the culture conditions are stable, the container has great sealing performance, and there is no alternation of hot and cold condition, the period of using can be appropriately extended, but not exceed 45 days.
Please use all products within the expiration date. Expired ingredients may significantly affect the cell culture effect.

Note: This product is intended for research use only and is not for diagnostic, therapeutic, clinical, household, or any other applications.

OriCell-HUXMX-90021-User-Manual.pdf View ››

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Stem Cells Dev 21:1418 (2012) IF = 3.727 　Year 2012
Toll-like receptors 2 and 4 mediate the capacity of human bone marrow-derived mesenchymal stromal cells to support the proliferation andd ifferentiation of CD34+ cells in a non-synergistic way.
Exp Cell Res 318:196 (2012) IF = 3.246 　Year 2012
Polycaprolactone electrospun mesh conjugated with an MSC afﬁnity peptide for MSC homing in vivo.
Biomaterials 33:3375 (2012) IF = 8.806 　Year 2012
Therapeutic potential of transplanted placental mesenchymal stem cells in treating Chinese miniature pigs with acute liver failure.
BMC Med BMCMed10:56 (2012) IF = 7.249 　Year 2012
Macrophage Migration Inhibitory Factor Inhibits the Migration of Cartilage End Plate-Derived Stem Cells by Reacting with CD74.
PLoS One 22952837 (2012) IF = 3.234 　Year 2012
Gene silencing of the BDNF/TrkB axis in multiple myeloma blocks bone destruction and tumor burden in vitro and in vivo.
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Senescence of bone marrow mesenchymal stromal cells is accompanied by activation of p53/p21 pathway in myelodysplastic syndromes.
Eur J Haematol 93:476 (2014) IF = 2.066 　Year 2014
Impaired osteogenic differentiation of mesenchymal stem cells derived from bone marrow of patients with lower-risk myelodysplastic syndromes.
Tumour Biol 35:4307 (2014) IF = 3.611 　Year 2014
Efficient Reprogramming of Naı¨ve-Like Induced Pluripotent Stem Cells from Porcine Adipose-Derived Stem Cells with a Feeder-Independent and Serum-Free System.
PLoS One PMID:24465482 (2014) IF = 3.234 　Year 2014
Pro-osteogenic effects of fibrin glue in treatment of avascular necrosis of the femoral head in vivo by hepatocyte growth factor-transgenic mesenchymal stem cells.
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Transplantation of Human Menstrual Blood Progenitor Cells Improves Hyperglycemia by Promoting Endogenous Progenitor Differentiation in Type 1 Diabetic Mice.
Stem Cells Dev 23:1245 (2014) IF = 3.727 　Year 2014
Bone marrow mesenchymal stromal cells affect the cell cycle arrest effect of genotoxic agents on acute lymphocytic leukemia cells via p21 down-regulation.
Ann Hematol 93:1499 (2014) IF = 2.634 　Year 2014
Angiopoietin-1 gene-modified human mesenhymal stem cells promote angiogenesis and reduce acute pancreatitis in rats.
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Adipose-derived stem cell-based treatment for acute liver failure.
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Application of a Novel Population of Multipotent Stem Cells Derived from Skin Fibroblasts as Donor Cells in Bovine SCNT.
PLoS One PMID:25602959 (2015) IF = 3.234 　Year 2015
FOXO1 inhibits osteosarcoma oncogenesis via Wntβ-catenin pathway suppression.
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Gastric cancer-derived mesenchymal stem cells_prompt gastric cancer progression through_secretion of interleukin-8.
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GFP Labeling and Hepatic Differentiation Potential of Human Placenta-Derived Mesenchymal Stem Cells.
Cell Physiol Biochem 35:2299 (2015) IF = 2.875 　Year 2015
Human adipose-derived mesenchymal stem cells repair cisplatin-induced acute kidney injury through antiapoptotic pathways.
Exp Ther Med 10:468 (2015) IF = 1.269 　Year 2015
Rho kinase inhibitor Y-27632 promotes the differentiation of human bone marrow mesenchymal stem cells into keratinocyte-like cells in xeno-free conditioned medium.
Stem Cell Res Ther PMID:25889377 (2015) IF = 4.963 　Year 2015
Surface modification on polycaprolactone electrospun mesh and human decalcified bone scaffold with synovium-derived mesenchymal stem cells-affinity peptide for tissue engineering.
J Biomed Mater Res A 103:318 (2015) IF = 3.369 　Year 2015
Activation and regulation of the granulation tissue derived cells with stemness-related properties.
Stem Cell Res Ther PMID:25925316 (2015) IF = 4.963 　Year 2015
MiR-708 promotes steroid-induced osteonecrosis of femoral head, suppresses osteogenic differentiation by targeting SMAD3.
Scientific Reports 6: 22599 (2016) IF = 4.259 　Year 2016
Effects of Intermittent Administration of Parathyroid Hormone (1-34) on Bone Differentiation in Stromal Precursor Antigen-1 Positive Human Periodontal Ligament Stem Cells.
Stem Cells Int PMID: 27069479 (2016) IF = 3.687 　Year 2016
Adipogenic placenta-derived mesenchymal stem cells are not lineage restricted by withdrawing extrinsic factors developing a novel visual angle in stem cell biology.
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Iron overload induced death of osteoblasts in vitro_involvement of the mitochondrial apoptotic pathway.
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Localization of human adipose-derived stem cells and their effect in repair of diabetic foot ulcers in rats.
Stem Cell Research & Therapy 7:155 (2016) IF = 4.211 　Year 2016
Adipogenic placenta-derived mesenchymal stem cells are not lineage restricted by withdrawing extrinsic factors: developing a novel visual angle in stem cell biology.
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Bone Morphogenetic Protein-9 Enhances Osteogenic Differentiation of Human Periodontal Ligament Stem Cells via the JNK Pathway.
PLoS One PMID: 28052093 (2016) IF = 3.057 　Year 2016
Porcine Adipose-Derived Mesenchymal Stem Cells Retain Their Stem Cell Characteristics and Cell Activities While Enhancing the Expression of Liver-Specific Genes after Acute Liver Failure.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES DOI: 10.3390/ijms17010062 (2016) IF = 4.183 　Year 2016
In vitro and in vivo study of additive manufactured porous Ti6Al4V scaffolds for repairing bone defects.
Sci Rep PMID:27667204 (2016） IF = 4.259 　Year 2016
MIF Plays a Key Role in Regulating Tissue-Specific Chondro-Osteogenic Differentiation Fate of Human Cartilage Endplate Stem Cells under Hypoxia.
Stem Cell Reports 7:249–262 (2016） IF = 6.537 　Year 2016
Silencing of Long Non-Coding RNA NONHSAT009968 Ameliorates the Staphylococcal Protein A-Inhibited Osteogenic Differentiation in Human Bone Mesenchymal Stem Cells.
Cell Physiol Biochem 39:1347-59 (2016） IF = 4.652 　Year 2016
Targeting of the leukemia microenvironment by c(RGDfV) overcomes the resistance to chemotherapy in acute myeloid leukemia in biomimetic polystyrene scaffolds.
ONCOLOGY LETTERS 12:3278-3284 (2016) IF = 1.482 　Year 2016
Effects of mesenchymal stem cells from human induced pluripotent stem cells on differentiation, maturation, and function of dendritic cells.
Stem Cell Res Ther 8:48 (2017) IF = 4.963 　Year 2017
Periostin Upregulates Wnt/β-catenin signaling to promote the osteogenesis of CTLA4-modified human bone marrow-mesenchymal stem cells.
Scientific Reports 7:41634 (2017) IF = 4.122 　Year 2017
Enhanced tumor growth inhibition by mesenchymal stem cells derived from iPSCs with targeted integration of interleukin24 into rDNA loci.
Oncotarget 8:40791-40803 (2017) IF = 5.168 　Year 2017
miR-29c-3p promotes senescence of human mesenchymal stem cells by targeting CNOT6 through p53–p21 and p16–pRB pathways.
Biochimica et Biophysica Acta——Molecular Cell Research 520–532 (2016) IF = 4.521 　Year 2016
Porphyromonas gingivalis can invade periodontal ligament stem cells.
BMC Microbiology 17:38 (2017) IF = 2.644 　Year 2017
Periostin Upregulates Wnt/β-Catenin Signaling to Promote the Osteogenesis of CTLA4-Modified Human Bone Marrow-Mesenchymal Stem Cells.
Sci Rep 7:41634 (2017) IF = 4.259 　Year 2017
Premature exhaustion of mesenchymal stromal cells from myelodysplastic syndrome patients.
Am J Transl Res 9:34623468 (2017) IF = 2.829 　Year 2017
ERK1/2 signaling mediated naringin-induced osteogenic differentiation of immortalized human periodontal ligament stem cells.
Biochemical and Biophysical Research Communications 489:319-325 (2017) IF = 2.466 　Year 2017
Mesenchymal stem cell-conditioned medium promotes MDA-MB-231 cell migration and inhibits A549 cell migration by regulating insulin receptor and human epidermal growth factor receptor 3 phosphorylation.
ONCOLOGY LETTERS 13:1581-1586 (2017) IF = 1.39 　Year 2017
Knockdown of SIRT7 enhances the osteogenic differentiation of human bone marrow mesenchymal stem cells partly via activation of the Wnt/β-catenin signaling pathway.
Cell Death Dis 8:e3042 (2017) IF = 5.638 　Year 2017
Multifunctional Copper-Containing Carboxymethyl Chitosan/ Alginate Scaffolds for Eradicating Clinical Bacterial Infection and Promoting Bone Formation.
ACS Appl Mater Interfaces 10:127-138 (2017) IF = 7.504 　Year 2018
Human colorectal cancer-derived mesenchymal stem cells promote colorectal cancer progression through IL-6/JAK2/STAT3 signaling.
Cell Death Dis 9:25 (2018) IF = 5.638 　Year 2018
Bioinspired fabrication of carbonated hydroxyapatite/chitosan nanohybrid scaffolds loaded with TWS119 for bone regeneration.
Chemical Engineering Journal DOI:10.1016/j.cej.2018.02.010 (2018) IF = 6.216 　Year 2018
IL-6 potentiates BMP-2-induced osteogenesis and adipogenesis via two different BMPR1A-mediated pathways.
Cell Death Dis 9:144 (2018) IF = 5.638 　Year 2018
Targeting skeletal endothelium to ameliorate bone loss.
Nat Med 24:823-833 (2018) IF = 32.621 　Year 2018
MiR-455-3p inhibits the degenerate process of chondrogenic differentiation through modification of DNA methylation.
Cell Death Dis 9:537 (2018) IF = 5.638 　Year 2018
Impact of Oxygen Concentration on Metabolic Profile of Human Placenta-Derived Mesenchymal Stem Cells As Determined by Chemical Isotope Labeling LC–MS.
J Proteome Res 17:1866-1878 (2018) IF = 3.95 　Year 2018
Long noncoding RNA lncAIS downregulation in mesenchymal stem cells is implicated in the pathogenesis of adolescent idiopathic scoliosis.
Cell Death Differ PMID:30464226 (2018) IF = 8 　Year 2018
Extracellular matrix deposited by Wharton’s jelly mesenchymal stem cells enhances cell expansion and tissue specific lineage potential.
Am J Transl Res 10:3465-3480 (2018) IF = 3.061 　Year 2018
Tumor-derived extracellular vesicles inhibit osteogenesis and exacerbate myeloma bone disease.
Theranostics 9:196-209 (2019) IF = 8.537 　Year 2019
Cajanolactone A from Cajanus cajan Promoted Osteoblast Differentiation in Human Bone Marrow Mesenchymal Stem Cells via Stimulating Wnt/LRP5/β-Catenin Signaling.
Molecules 24 (2019) IF = 3.098 　Year 2019
Expression of exosomal microRNAs during chondrogenic differentiation of human bone mesenchymal stem cells.
J Cell Biochem 120:171-181 (2019) IF = 2.959 　Year 2019
Extracellular vesicles from human urine-derived stem cells prevent osteoporosis by transferring CTHRC1 and OPG.
Bone Research 7:18 (2019) IF = 12.354 　Year 2019
PLEXIN-B2 promotes the osteogenic differentiation of human bone marrow mesenchymal stem cells via activation of the RhoA signaling pathway.
Cellular Signalling PMID: 31176746 (2019) IF = 3.487 　Year 2019
Molecular mechanism underlying the difference in proliferation between placenta-derived and umbilical cord-derived mesenchymal stem cells.
Journal of Cellular Physiology PMID: 31990045 (2020) IF = 4.522 　Year 2020
The proangiogenic effects of extracellular vesicles secreted by dental pulp stem cells derived from periodontally compromised teeth.
Stem Cell Research & Therapy PMID: 32143712 (2020) IF = 4.627 　Year 2020
The lncRNA Rhno1 miR-6979-5p BMP2 Axis Modulates Osteoblast Differentiation.
International Journal of Biological Sciences Doi: 10.7150/ijbs.38930 IF = 4.067 　Year 2020
Curcumin Inhibits Calcification of Human Aortic Valve Interstitial Cells by Interfering NF-κB, AKT, and ERK Pathways.
PHYTOTHERAPY RESEARCH PMID: 32189385 (2020) IF = 3.766 　Year 2020
Three-dimensional Culture of MSCs Produces Exosomes With Improved Yield and Enhanced Therapeutic Efficacy for Cisplatin-Induced Acute Kidney Injury.
Stem Cell Res Ther DOI: 10.1186/s13287-020-01719-2 IF = 4.627 　Year 2020

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