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Osteoclast Differentiation Medium For RAW264.7
Introduction
OriCell™ Osteoclast Differentiation Medium For RAW264.7 (Basic Version) is meticulously developed by our R&D team for the differentiation of RAW 264.7 cells into osteoclasts. The kit consists of a specialized differentiation medium, essential supplements and staining solutions, providing a complete solution for osteoclast differentiation.
Extensive validation studies have demonstrated that this product can stably and efficiently induce the differentiation of RAW 264.7 cells into osteoclasts in vitro. The optimized formulation supports reproducible differentiation outcomes and consistent osteoclast formation, facilitating reliable downstream applications in bone biology, osteoimmunology, and drug discovery research.
When citing our products in academic publications, please use the following format: "OriCell™ [Product Name] + [Catalog Number], from Cyagen Biosciences."
Product Information
| Components of Medium Kit | Catalog Number | Volume | Storage |
| OriCell™ Basal Medium For Cell Culture | BAME-03011 | 45 mL | 4 ℃ |
| OriCell™ Supplements For Osteoclast Differentiation I | CLROC-04401 | 5 mL | -20 ℃ |
| OriCell™ Supplements For Osteoclast Differentiation II | CLROC-04402 | 25 μL | -20 ℃ |
| OriCell™ Supplements For Osteoclast Differentiation III | CLROC-04403 | 12.5 μL | -20 ℃ |
| Components of Staining Solutions | Catalog Number | Volume | Storage |
| OriCell™ TRAP Staining Solution Component I | TRAP-10001 | 1 mL | -20 ℃ |
| OriCell™ TRAP Staining Solution Component II | TRAP-10002 | 100 μL | -20 ℃ |
| OriCell™ TRAP Staining Solution Component III | TRAP-10003 | 9 mL | 4 ℃ |
QC
- Pass the detection of bacteria, fungi, mycoplasma, and endotoxins.
- Pass the detection of osmotic pressure and pH.
- Pass the detection of product quality.
Please refer to "COA" for details.
General Handling Principles
- Maintain strict aseptic technique. Ensure complete sterility throughout all procedures, particularly within the laminar flow hood and incubator.
- Follow standardized protocols. Adhere strictly to the product manual instructions. Implement rigorous control over experimental variables and include appropriate parallel controls.
- Ensure proper storage and use. Store all components according to specified conditions and use them promptly to ensure optimal performance.
- Aliquot for long-term storage. If the entire volume will not be used up immediately, prepare the medium in batches according to the specified component volume ratios and store in aliquots.
Product Stability and Storage Conditions
- All components must be stored away from light.
- The basal medium has a shelf life of 1 year and should be stored at 4 °C. All supplements have a shelf life of 1 year and should be stored at −20 °C. The staining solutions have a shelf life of 6 months and should be stored at 4 °C (I & II) and -20 °C (III) separately.
- Once prepared, the medium has a shelf life of 1 month when stored at 4 °C.
- Use all components before the expiration dates. Expired components may severely compromise culture performance.
